SnapGene

The Industry’s Most Popular Molecular Cloning Tool. SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures. The streamlined interface supports a range of cloning and PCR manipulations. SnapGene is the most popular cloning tool for a reason. It’s fast, smart and extremely user-friendly.

 

Improve accuracy while moving faster, so you save time and money.

• Elegant, information-rich windows for simulating common cloning and PCR methods

• Clear visual schematics let you see exactly how your construct will be put together

• SnapGene helps you identify and avoid common mis-steps by keeping track of details like DNA methylation and phosphorylation

 

Beyond the Basics of Molecular Cloning

• Intuitive technology identifies design flaws in cloning procedures so they can be corrected

• Simulate standard PCR using your own primers, or allow SnapGene to design them automatically

• Specialised cloning tools ensure fast accurate construct design for all major molecular cloning techniques

 

Restriction Cloning

Restriction cloning is a common cloning technique where restriction enzymes are used to prepare an insert and a vector for ligation.

 

Gateway Cloning

Plasmids can be constructed without restriction enzymes using Gateway cloning, which inserts DNA fragments by recombination. SnapGene simulates different variations of this method.

 

For standard Gateway cloning, a DNA fragment is inserted into a Donor Vector in a BP cloning reaction, creating an Entry Vector. Then the fragment is transferred to a Destination Vector in an LR cloning reaction, creating the final Expression Vector. Multisite Gateway cloning allows up to four fragments to be inserted simultaneously.

 

Gibson Assembly

Gibson Assembly is a popular way to insert fragments into a plasmid without using restriction enzymes. To simulate this method, SnapGene provides an intuitive interface.

 

For Gibson Assembly, PCR amplify the DNA segments to create overlapping